Abstract:..High-tech Zone is an important carrier to promote the progress of making China a country of innovators. In order to explore the influencing mechanism of constructing High-tech Zone, based on the questionnaire survey data from innovative enterprises, universities, government, and etc. from Donghu High-tech, this paper builds an influencing mechanism model which contains collaborative innovation environment, mechanism, modes, and performance by using the methods of SEM and regression. Empirical research shows that the collaborative innovation environment, mechanism, and modes have a significant positive correlation with collaborative innovation performance, the collaborative innovation mechanism plays a mediator role in the relationship between collaborative innovation environment and performance, and the collaborative innovation modes (i.e. strategic alliance, technology patents, R D outsourcing, and collaborative elements transfer) play positive moderating role between the collaborative innovation environment and performance.