Abstract:The "spatiotemporal compression effect" of the Internet has fully unlocked the knowledge resources of space and become an important breach of knowledge spillover and regional innovation. Based on the provincial innovation data of China from 2007 to 2018, this paper measures the evolution characteristics of regional innovation space, constructs a knowledge production function that comprehensively considers Internet popularization and knowledge spillover, and empirically analyzes the impact of Internet popularization and knowledge spillover on the evolution of regional innovation space using the spatial Dubin model. The results show that there is a great imbalance in the innovation space among provinces in China, and the differences between provinces show an inverted "U" pattern with 2012 as the dividing line, including that ①The popularization of the Internet has a significant inhibitory effect on regional innovation output in this region; ②The knowledge stock spillover has a significant positive effect on regional innovation output, while the knowledge quality spillover presents an opposite effect; ③The popularization of the Internet amplifies the impact of knowledge spillover on regional innovation output level. The results of this study are helpful to fully understand the feasible path of spatial evolution of regional innovation and have important practical significance to realize coordinated development.