Abstract:This paper analyzes ?the present research situation of entrepreneurial?university, evolution of university-industry?cooperation(UIC), iterated prisoners dilemma (IPD)?and its evolution strategy at?first. then?based?on?the asymmetric IPD game and simulation platform Netlogo, builds the Multi-Agent simulation?model of Evolution of Cooperative Behavior between Entrepreneurial Universities and Enterprises , introduces the classical evolutionary strategy “TIT FOR TAT” (TFT) , carries a deep analysis on a?positive?role of TFT?in?promoting? Behavioral Evolution of the UIC,With application of the classical evolutionary strategy TFT and other conditions remaining unchanged, research result shows that the evolution of cooperative behavior is uncorrelated with corporate collaborators density, positively correlated with participants’ numbers, negatively correlated with the space scope, the influence of revenue-sharing?coefficient is of nonlinear?characteristics. Finally in this basis, respectively from the game?body and the government level it gives some specific countermeasures and suggestions. This research has practical significance in avoiding the?prisoner's?dilemma in UIC and expanding?the?range?of?studies on the IPD game.