Abstract:Based on the analysis of the main influencing factors of the performance of knowledge flow and the relationship between these factors during industry-university cooperation, the system dynamics model, which was running with Vensim PLE software to simulate this system, was set up in order to analyze how trust, knowledge sharing and information symmetry affect knowledge flow performance. The results of simulation show that trust, knowledge sharing and information symmetry have an obviously positive impact on the performance of knowledge flow, and the effect of knowledge sharing and information symmetry on the performance of knowledge flow is more significant; trust, knowledge sharing and information symmetry have a more significant impact on the universities’ technology transferring income than on the number of projects, to which research and development results have been applied.