Abstract:In recent years, science and technology business incubator(“Incubator” for short) construction boom across the country, all kinds of background and patterns such as incubators emerged like bamboo shoots after a spring rain, under the new situation, to explore the construction of the incubator operations and a new profit model for an effective attempt. Since the establishment of the first national incubator from 1987 Wuhan East Lake, throughout the course of development of 30 years, from the beginning of the incubator to space rental and the 1.0 mode to the 3.0 mode in the current shareholding incubator, providing services for entrepreneurs to the depth and breadth are constantly expanding. However, these incubators also can not meet the needs of entrepreneurs, but also can not solve their own survival and development of common problems. This paper puts forward the concept of the 4.0 mode of incubator development, combined with the practical cases conducted a preliminary research on the new definition, new trends, new models, new sources, and puts forward some suggestions to promote the development of the 4.0 mode of incubator.