Abstract:Entrepreneurial opportunity identification is the core and starting point of entrepreneurship.This article analyse the influence of prior knowledge Entrepreneurial Alertness and Creative thinking for entrepreneurial opportunity identification.The article also demonstrate the mediating effect of Entrepreneurial Alertness and Creative thinking . .The results showed that prior knowledge, entrepreneurial alertness and creative thinking all make significant positive effects on entrepreneurial opportunity identification, and the impact of entrepreneurial alertness on entrepreneurial opportunity identification is the most significant. Different from the previous research results, this study has a significant positive impact on all dimensions of the independent variables. Finally, according to the research results of system analysis and summary of research results, we put forward the policy suggestion and reference direction for entrepreneurs and government and education organizations, entrepreneurs or managers should pay attention to the cultivation of their own interests and through entrepreneurial learning enrich their industry knowledge. The school should all increase related industries and practice knowledge of the depth and breadth of entrepreneurship education and entrepreneurial training and focus on strengthening the cultivation of students" creative thinking; the government should strengthen relevant policies to support entrepreneurship efforts to further improve the recognition ability of entrepreneurs or managers of the entrepreneurial opportunities.