Abstract:This paper is listed on the Shenzhen Stock Exchange in 2012-2015, 463 advanced technology enterprises are used the research samples. Based on panel data, the paper analysis empirically that Internal control moderates the relationship between R&D investment and enterprise performance. The results show that the current period and a phase lag R&D investment have significant negative influence on enterprise performance; Two phase lag R&D investment has significant positive influence on enterprise performance. Internal control can improve the enterprise performance. Internal control has a negative regulatory effect on the current and lag of R&D investment and enterprise performance relationship.