Abstract:Job insecurity will bring serious impact to the organization and employees both. Academic researches on job insecurity mostly focus on the study of outcome variables, while ignoring the antecedent variables. The purpose is to explore the job insecurity and the consequences of knowledge workers by exploring the influencing factors and mechanism of job insecurity. An empirical analysis of 219 valid data was performed by software such as Lisrel and SPSS, the empirical results show that job insecurity significantly affects the turnover intention and subjective well-being of knowledge workers. Job uncertainty is a predictor of job insecurity. Organizational support for development can effectively reduce job uncertainty and job insecurity as a stress intervention strategy. Meanwhile, it confirms the chained mediation effect of job uncertainty and job insecurity between organizational support for development and turnover intention or subjective well-being of knowledge workers. It enriches the domestic research on job insecurity, and provides reference for enterprise management in the current changing situation.