Abstract:Some research identifies the intra-urban Co-Opetition relationship has become a key factor in the development of urban agglomerations. Meanwhile, innovation driving plays an important role in promoting the transformation and upgrading of urban industry,economic sustainable development. This paper uses the 27 categories of manufacturing data to analysis Co-Opetition relationship between Dongguan and other cities. The results show that Dongguan and Guangzhou, Shenzhen, mainly keep the mutual conversion between the strong complementary relations of cooperation and strong competition; however, it mainly keeps the stable relationship with other six cities. Also, the evolution of the relationship shows the polarization is more obvious. Under the drive of innovation strategy, it is believed that Optimizing the pattern of industrial division in Dongguan and establishing a coordinated mechanism for regional economic development are to strengthen economic cooperation between Dongguan and other cities of Pearl River Delta urban agglomeration, to achieve Dongguan sustainable economic development and to take important steps in the transformation and upgrading of manufacturing industry.