Abstract:The purpose of this study is to discuss the structure of scientific and technological innovation talents? entrepreneurial competence who starting businesses in cross-border e-commerce and the cultivation mechanism of entrepreneurial competence which based on three triple helix innovation drive. On the basis of literature review, interviews and case studies, author proposed the hypothesis model of scientific and technological innovation talents? entrepreneurial competence structure as well as the cultivation mechanism of entrepreneurial competence. Finally, through the questionnaire study of 183 scientific and technological innovation talents who come from eight cross-border e-commerce pilot cities include hangzhou, Shanghai, Ningbo, Chongqing , Guangzhou , Zhengzhou, Tianjin , Shenzhen to verify and correct the hypothesis model. From the research we can conclud that scientific and technological innovation talents entrepreneurial competence includes entrepreneurial opportunities competency, cross-border cultural competence, team building competency, business innovation competence and entrepreneurial performance competence. Entrepreneurial competence cultivation mechanism can be builded from the government"s policy driven , entrepreneurship education in colleges as well as industry collaborative training. The three are linked and working together to affect entrepreneurial competence. The element of cultivation mechanism have different effects on the different dimensions of the talents? entrepreneurial competence.