Abstract:Large amount of research and empirical work have been done on the relationship between R&D investment and Enterprise performance by domestic and international scholars. However, most of the existing works focus on qualitative research of their relationship and the direct and indirect interactive effects affecting the relationship, which mainly came up with qualitative conclusion. A small number of scholars applied quantitative analysis to express the mathematical relationship between the relationship between R&D investment and Enterprise performance, but in terms of the large number of influencing factors, this quantitative analysis method has its own limitation. In order to explore a method of quantitative analysis of a large number of factors at the same time, this paper tries to analyze 327 manufacturing enterprises in stock markets of Shanghai and Shenzhen in 2011~2016 by using the analysis method of big data. The analysis target is set as more than zero the growth of R&D input and revenue per capita income to get quantitative optimization rules.