Abstract:For the knowledge workers with high quality and high pursuit, the influence of traditional motivation factors has gradually declined, and the inner sense of mission can drive them to improve their work performance and achieve job innovation. Based on the situation in China, this paper investigates 207 knowledge workers in knowledge-intensive enterprises, selects career call as the main research variable, and explores the relationship between career call and employee innovation behavior through empirical analysis. The influence and role of organizational support in this relationship and the influence of external environmental factors on employees' internal motivation are also discussed. The results show that career call has a significant positive effect on innovation behavior and its subordinate innovation conception and innovation execution,organizational support sense negatively regulates the positive relationship between career call and innovation behavior and its dimensions. The result verifies the theory of career call in Chinese context and its influence on the innovative behavior of knowledge workers, and provides a reference for the practice of talent incentive management in enterprises.