Abstract:Against the strategy choice of government and enterprises in the ecological environment protection of the Yangtze River Economic Belt in the context of "The sewage charges system was changed to the environmental protection tax system" in environmental protection, the evolutionary game model for ecological environment protection government regulation in the Yangtze River Economic Belt was constructed. It reveals the change of strategy between government and enterprises. Research results show that the cost of government supervision, the increased cost of legal waste discharge by enterprises, the increase in profits from legal waste discharge, and the penalties imposed on enterprises for illegal discharge of pollutants have important influence on the strategic choices of government and enterprises. On this basis, we put forward some countermeasures and suggestions. They are establishing a coordinated protection mechanism for the ecological environment of the Yangtze River Economic Belt, giving full play to the economic leverage adjustment of environmental protection tax, Implementing the strictest ecological environment protection system in the Yangtze River Economic Belt and so on.