Abstract:Cluster supply chain is an emerging supply chain model under the backgrou-nd of industrial clusters, which is of great significance to the development of regional economy. Knowledge, as the company's core production data, is one of the important factors that affect the overall operational efficiency of the supply chain. In order to promote the development of regional economy and enterprises, the research on the knowledge sharing behavior of cluster supply chain is studied. This paper uses evolutionary game theory to establish the evolutionary game model of knowledge sharing behavior among horizontal enterprises in cluster supply chain, and analyzes the evolution path of knowledge sharing behavior in cluster supply chain and the characteristics of enterprises that affect knowledge sharing behavior. The research results show that the knowledge sharing behavior of cluster supply chain is mainly affected by the characteristics of the sharing of cost, sharing risk, marginal benefit, knowledge absorption capacity, the amount of knowledge sharing, incentive coefficient and penalty coefficient; reducing share cost and risk and improving marginal benefit ,the ability to absorb knowledge and the amount of knowledge sharing, and the introduction of incentive and punishment mechanisms, will increase the willingness of enterprises in the supply chain of the cluster to choose for knowledge sharing.