Abstract:The construction of world-class universities and first-class disciplines is an important development strategy in China''s current higher education field. ESI is one of the most important evaluation index tools commonly used worldwide to evaluate the academic level and impact of universities, academic institutions, as well as the countries and regions. In this paper, based on the ESI database from 2007 to 2017, the domestic and overseas universities with the top ranking in ESI system of agricultural science are focused on and analyzed. Multiple index including the amount top papers, highly cited papers, hot papers as well as citation number of total papers, the average in per paper and research fronts are compared to evaluate the performance of scientific research objectively, providing the reference to the performance evaluation of "double first-class" construction.