Abstract:" Made in China 2025" provides policy guidance for the sustainable development road of green manufacturing in China. Based on the co-evolution background of green manufacturing and green logistics, the innovation of enterprise knowledge chain must be studied under the background of industrial development, so as to ensure the innovation vitality of enterprise knowledge chain and avoid the restriction of knowledge link path dependence. At the same time, it can further develop and improve the theoretical research of enterprise knowledge chain. On the basis of studying and analyzing the connotation and limitation of knowledge chain, this paper discusses the necessity of enterprise knowledge chain innovation under the co-evolution of green manufacturing and logistics. Secondly, the feasibility of solving knowledge link path dependence from information structure is analyzed. Based on the collaborative background of green manufacturing and logistics, a feedback loop model of knowledge chain is constructed, and corresponding innovative countermeasures are proposed.