Abstract:With the globalization of research and development, the international scientific and technological innovation cooperation of China''s research universities is also increasing. By means of investigation and data analysis, this paper discusses the status and development trend of scientific and technological innovation cooperation between research universities and multinational enterprises in the context of globalization, analyzes the operational mechanism of scientific and technological innovation cooperation, and sums up the typical model packages of scientific and technological innovation cooperation between research universities and multinational enterprises in China. It includes three aspects: technology research and development, transformation and transfer of scientific and technological achievements, and talent exchange and training.This paper studies 11 key factors that affect the effectiveness of the cooperation in scientific and technological innovation, and takes the cooperation in technological research and development as an example, analyzes the strategy of choosing the cooperation mode, and puts forward relevant suggestions on the international cooperation in scientific and technological innovation between China''s education authorities and universities.