Abstract:The scientific and technological innovation teams often use a single measure method, such as the difference between objectives and performance, to evaluate their performance, which often has some problems, because the basic conditions used in setting goals often change when the target is implemented, some biases in setting the target value may lead to optimization of some of the target values. In order to better measure the performance of the a scientific and technological innovation team, the difference between the profit based on the best operational action under actual conditions and best profit based on the actual value and the benchmark value is derived, that is, the method of evaluating the performance of science and technology innovation team based on opportunity loss. The results show that:it is necessary to use the principles and methods of overall best profit and partial best profit, total profit and overall opportunity loss and partial opportunity loss to measure the performance of scientific and technological innovation team based on opportunity loss; in addition, in the case of plural scientific and technological innovation teams, two parts of the best profits need to be considered, at which time there will be four partial loss of opportunity. The paper further discusses and explains the principles and methods of two scientific and technological innovation team performance measurement based on opportunity loss.