Abstract:In addition to explicit knowledge, tacit knowledge is also an important resource for the innovation and development of science and technology enterprises.Combined with the evolutionary game theory, this paper analyzes the factors and mechanisms that affect the tacit knowledge flow by using the replicator dynamics equation, and concludes that the degree of tacit knowledge flow in technology enterprisesis determined by the cost of tacit knowledge dissemination, the incentive of technology enterprises to the tacit knowledge communicators, and the income generated by two-way knowledge flow and one-way knowledge flow, etc. From the point of promoting the mechanism of the tacit knowledge flow of enterprises, this paper puts forward some thought methods, such as establishing internal and external knowledge alliance, strengthening knowledge management and encouraging, to link and overcome the problem of "island of knowledge" of organizational knowledge flow.