Abstract:Smart Specialization provides new development opportunities for regional innovation and development. As a new force in the construction of innovation ability, universities have a profound impact on the formulation of wisdom and specialization strategy. In this paper, through the construction of a comprehensive evaluation index system of scientific and technological innovation ability of universities, factor analysis method is used to compare and analyze the scientific and technological innovation ability of universities in the eastern, central and western provinces of China. The results show that the comprehensive ability of scientific and technological innovation in universities is decreasing from the east to the West. This paper further analyzes the input-output capability and output capability, and divides 31 provinces into three types: input-output coordination type, input leading type and output leading type. When promoting the balanced development of regional wisdom and specialization, universities in the central and western regions can establish a compensation mechanism for scientific research resources and deepen the cooperation between industry, university and research institute to improve the scientific and technological innovation ability. In addition, the output leading provinces should increase the input of innovation resources, and the input leading provinces should carry out bilateral reform of supply and demand to improve the output and transformation strength of scientific and technological achievements.