Abstract:China"s large enterprises are the main body of scientific and technological innovation, and their spatial distribution has certain regularity. Based on the micro data of China"s top 500 manufacturing and service enterprises from 2005 to 2017, this paper reveals the relative feature of their location evolution, and also tests whether there is "interdependence" between them in the geographical spatial distribution pattern by using the barycenter model, hotspot analysis and other spatial methods. Then, the impact of service industry on the location selection of manufacturing enterprises is analyzed by using the negative binomial regression model. The results are as follows. Firstly, the hot spots and sub hot spots of the two types of corporate headquarters are moving southward. The Yangtze River Delta is the common hot spots of the two types of corporate headquarters. Secondly, the gravity of manufacturing and service enterprises is moving southward, and the distance between the gravity of two types of corporates is gradually reduced. The correlation degree of the gravity variation is enhanced. Thirdly, manufacturing enterprises tend to be located in areas with high level of high-end producer services, in which financial service, science and technology service, transportation service have a significant impact on the location of the top 500 manufacturing enterprises.