Abstract:it is very important for an enterprise to innovate its business model to adapt to the changes of external environment. Based on the dualistic theory, this paper discussed the impact of ambidextrous innovation on business model innovation and the moderating role of organizational routine updating. Taking 276 enterprise survey data as samples, the research results show that disruptive innovation has a significant positive impact on business model innovation. Meanwhile, incremental innovation is beneficial to the innovation of efficient business model, but hinders the innovation of value business model. Then the balance and complementarity of ambidextrous innovation have positive effects on business model innovation. At last, the organizational routine updating moderates the relationship between ambidextrous innovation and business model innovation.