Abstract:This study has used the literature measurement analysis method, using CiteSpaceⅤ from 2000 to 2020 Chinese knowledge network, data of ten thousand and Chongqing weipu journals included in CSSCI database, core journal of Peking University and the Chinese science citation database (CSCD), published in the literature about regional innovation ecosystem is analyzed, explored the regional innovation ecosystem research status and development trend. The results show that the research of regional innovation ecosystem in China has regional characteristics and there is less cooperation among different research institutions. The research focus of regional innovation ecosystem in China mainly focuses on the symbiotic evolution and niche of ecosystem. It is an important trend and direction of regional innovation ecosystem research in the future to combine regional characteristics, ecological characteristics and multi-source heterogeneous data.