Abstract:Little research has been done on the mechanism and boundary conditions of the interaction between industry, university and research institute as an external mechanism and the absorptive capacity of knowledge as an internal mechanism. This paper obtains 325 first-hand valid data through questionnaire survey to carry on the empirical research, the research conclusion has confirmed the research hypothesis, the conclusion shows that environmental turbulence, strategic consistency and structural formalization positively regulate the relationship between the cooperation of industry, university and research and the ability of knowledge absorption, which plays an intermediary role between the cooperation of industry, university and research and innovation performance. The research results have enriched the research literature on the spirit of "Deep integration of industry, university and research" , and opened the "black box" of the mechanism between the cooperation of industry, university and research and the absorptive capacity of enterprise knowledge on enterprise innovation performance, it provides practical enlightenment and countermeasures to promote enterprises to carry out the cooperation of industry, university and research and to promote the ability of absorbing knowledge.