Abstract:order to discover the influence of strategic differences among members of knowledge chain on Knowledge Synergy. Based on the principle of synergetics, with the target synergetic, benefit synergetic, resource synergetic and organizational structure synergetic as the prepositive variables of strategic synergetic, the influence model of knowledge chain strategic synergetic on knowledge synergetic is constructed, and the internal relation between them is explored and empirically tested by structural equation. The results show that the knowledge chain strategic synergy takes goal synergy, benefit synergy, resource synergy and organizational structure synergy as the pre variables to balance the strategic differences among the subjects of the knowledge chain and promote the realization of Knowledge Synergy in the knowledge chain.