Abstract:The factors that influence talents agglomeration are interwoven and interdependent, and have obvious configuration characteristics, therefore this paper focuses on the multi-dimensional elements of the innovation ecosystem and their linkage effects,based on the five helix innovation ecosystem theoretical framework, takes 31 provinces in China as the case samples, uses fuzzy set qualitative comparative analysis and necessary condition analysis to explore the complex causal mechanism of the configuration effect of the five elements of the innovation ecosystem, namely government, enterprises, universities, public and natural environment on regional talents agglomeration, and clarify the path that promotes the high agglomeration of talents and the path that leads to the non-high agglomeration of talents.The results show that there is no single necessary condition for high agglomeration of talents.?The contribution levels of all factors required for talents agglomeration varies greatly. From high to low, they are the government, universities and research institutes, enterprises, natural environment and the public, among which the public and the natural environment are basically equivalent.?There are three ways to drive the high agglomeration of talents,which are inclusive innovation-driven, government-industry-university-research collaboration and endogenous growth.?While There are two ways to lead to non-high agglomeration of talents,namely weak support from the government and the public and lagging development of enterprises.Based on the research findings, finally, some countermeasures and suggestions of promoting regional talents agglomeration are put forward from four aspects: "making up the short board+forging the long board", "government guidance+platform empowerment", "upgrading public service+active public participation" and "first-class universities as the core+government-industry-university-research collaboration".