Abstract:The collaborative agglomeration between environmental protection industry(EPI) and manufacturing industry is a symbiotic relationship with positive externalities at the industry level, and the relationship is significantly influenced by the policy sensitivity. Based on the perspective of industrial symbiosis evolution, This paper adopts the improved L-V model describing the process of EPI and manufacturing industry agglomeration and evolution. At the same time we descripts the path and characterization of th two industry in short-term dynamic evolution, as well as the role of environmental regulation in the process, with indroducing Environmental regulation into the model as the main influencing variable. The results show that the environmental regulation has a certain inhibitory effect on the manufacturing industry and a significant promoting effect on EPI, and it has a transfer effect on the symbiotic industry, and the Porter hypothesis is valid in the short-term. It is to be specific that the promotion effect of regulation on EPI has a dominant range. In this interval, the promotion effect basically reaches the peak, meanwhile, the impact on manufacturing industry is within the acceptable range. It is not conducive to the maximization of its incentive effect of the regulation level in too high or too low. The present elvel of environmental regulation is close to the optimal interval, and therefore regulation can be appropriately increased to make it enter the optimal interval. However, if we want to further improve the regulation level after reaching the optimal interval, It is necessary to carefully weigh the gains of the growth rate in EPI and the losses in manufacturing industry, and to consider in a comprehensive way in order to prevent the volatility effect of excessive regulation level.