Abstract:The Belt and Road providing an important channel for knowledge flow among countries along the Belt and Road. Studying knowledge influence of countries in the Belt and Road cooperation region can help to improve the quality of cooperation and knowledge innovation capacity. In this paper, the characteristics evolution of the nodes in national knowledge flow network which is constructed by cooperation relationship between countries along the Belt and Road. Knowledge centrality, knowledge intermediary and knowledge global are selected to evaluate the knowledge influence of countries in the cooperation region, and the formation reasons of knowledge influence are further explored. The study found that China leads the knowledge influence of countries along the Belt and Road by a large margin, making important contributions to the knowledge output and flow of other countries. National economic level, scientific research strength, openness degree and average social distance have significant influence with national knowledge influence, while geographical distance and cultural distance have no significant influence with national knowledge influence.