Abstract:The national laboratory is a strategic scientific and technological force that embodies the national will, realizes the national mission and represents the national level. On the basis of describing the management and operation mechanism of the United States Department of Energy National Laboratories, this paper introduces the theory of actor network, analyzes the composition of network actors and the network translation process of the National Laboratory from a sociological point of view, explains the interaction between actors and the formation mechanism of the interests alliance of the laboratory network, and puts forward countermeasures and suggestions for the management and operation of China"s national laboratory, such as attaching importance to the leading role of the core actors of laboratory personnel, strengthening the supporting role of all actors, and building a diversified governance mechanism for laboratories.