Abstract:Based on the entrepreneurial ecosystem theory, this paper explores the connotation of the entrepreneurial ecosystem of science and technology business incubators, proposes that the entrepreneurial ecosystem of science and technology business incubators consists of three parts: the entrepreneurial ecosystem leading population, the entrepreneurial ecosystem base population and the regional entrepreneurial ecological development environment, and constructs the evaluation index system of the incubator ecosystem. Using the entropy method, the incubator level of Beijing, Shanghai and Shenzhen from 2014 to 2021 was empirically analyzed. The results showed that the level of incubator ecosystem in the three cities increased year by year, with the largest increase in Shanghai; The level of incubator ecosystem in Beijing was the highest, followed by Shanghai and Shenzhen. The differences of incubator ecosystems in the three cities mainly focus on entrepreneurial enterprises, venture capital institutions, intermediary services, and enterprise environment. Finally, the author put forward some improvement measures, such as making continuous efforts to promote the development of incubators, strengthening the construction of key areas of incubators, strengthening the weak points to enhance the demonstration effect of incubators, and building an efficient collaborative innovation incubation ecology.