Abstract:At present, the research on the influence and mechanism of the Civil Code of the People's Republic of China, a major institutional reform and the vitality of scientific and technological innovation, is still lacking. In order to further clarify the impact degree and mechanism of the implementation of the Civil Code of the People's Republic of China and regional science and technology innovation, focus on the interactive relationship between the implementation of the Civil Code of the People's Republic of China and the vitality of regional science and technology innovation, and promote the development of science and technology innovation by discussing the static protection of rights and dynamic protection of transaction security of the civil code, based on the relevant data of 292 cities at the prefecture level and above in China from 2018 to 2020, Probit regression model was used to empirically test the effect and mechanism of the Civil Code of the People's Republic of China on regional scientific and technological innovation. The results show that the promulgation and implementation of the Civil Code of the People's Republic of China have a positive effect on the increase of the number of patent applications and patent grants in cities, and then promote regional scientific and technological innovation; The specific mechanism is to improve the level of regional scientific and technological innovation by optimizing the urban business environment, and the optimization of the business environment is especially reflected in the level of improving competition fairness. In order to consolidate the positive effect of the implementation of the Civil Code of the People's Republic of China, it is necessary to further improve the top-level legislative design and legal implementation mechanism, enhance the publicity, improve the evaluation system, and promote the vitality and momentum of regional scientific and technological innovation.